{
    "mmrrc_id": "44",
    "strain_name": "B6;129/Sv <i>Amelx<sup>tm1Kul</sup></i>/Mmnc",
    "rrid": "RRID:MMRRC_000044-UNC",
    "status": "Accepted",
    "accepted_dt": "2002-02-26",
    "delayed_release_requested": "0",
    "ilar_lab_code": "Kul",
    "published_name": "",
    "is_transgenic": "No",
    "founder_background": "(129X1/SvJ x 129S1/Sv)F1-Kitl<sup>+</sup>",
    "hom_fertile_comment": "",
    "is_sexlinked": "No",
    "genetic_alteration": "A neomycin selection cassette replaced 300 bp of the <i>Amelx </i>exon 2/intron 2, including the translation start site.",
    "phenotype": "Abnormal teeth with chaulky-white discoloration, attrition of cuspal tips associated with enamel hypoplasia.",
    "strain_development": "R1 ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were backcrossed to C57BL/6 females to generate heterozygotes, siblings of which were inbred to develop homozygous animals.",
    "availability_level": "",
    "center_id": "UNC",
    "assigned_dt": "2002-02-26",
    "availability": "Cryo-archive",
    "locus": [
        {
            "mmrrc_id": "44",
            "alteration": "Knockout",
            "alteration_id": "8",
            "additional_notes": "",
            "cell_line_id": "9",
            "symbol": "Amelx<tm1Kul>",
            "segment_type": "Allele",
            "species": "Mus musculus (mouse)",
            "locus_id": "6",
            "mgi_acc_id": "MGI:2177294",
            "locus_role": "Target",
            "chromosome": "X",
            "name": "amelogenin, X-linked; targeted mutation 1, Ashok B Kulkarni",
            "external_acc_id": "MGI:2177294",
            "resource_id": "MGI",
            "markup_symbol": "Amelx<sup>tm1Kul</sup>"
        },
        {
            "mmrrc_id": "44",
            "alteration": "Knockout",
            "alteration_id": "8",
            "additional_notes": "",
            "cell_line_id": "9",
            "symbol": "Amelx",
            "segment_type": "Gene",
            "species": "Mus musculus (mouse)",
            "locus_id": "5",
            "mgi_acc_id": "MGI:88005",
            "locus_role": "Target",
            "chromosome": "X",
            "name": "amelogenin, X-linked",
            "external_acc_id": "MGI:88005",
            "resource_id": "MGI",
            "markup_symbol": "Amelx"
        }
    ],
    "strain_of_origin": "(129X1/SvJ x 129S1/Sv)F1-Kitl<sup>+</sup>",
    "background_strain": "STOCK",
    "product": {
        "prod_hom_f": "1",
        "prod_hom_m": "1",
        "prod_het_hemi_f": "1",
        "prod_het_hemi_m": "1",
        "prod_sperm": "0",
        "prod_embryos": "0",
        "prod_resusc": "1",
        "prod_wt_control_f": "1",
        "prod_wt_control_m": "1",
        "prod_es_cell_line": "0"
    }
}