{ "mmrrc_id": "67", "doc_title": "MMRRC:000067-MU Detail Page", "published_name": "", "genetic_alteration": "This transgene contains the promoter of the mouse beta myosin heavy chain (myosin, heavy polypeptide 7, cardiac muscle, beta) gene, in a ~5.6 kb genomic DNA fragment encompassing upstream sequence and the entire 5-untranslated region (5-UTR) to the translation initiation site in exon 4, driving expression of a 603-bp mouse phospholamban cDNA fragment including 13 bp of the 5-UTR, the full-length protein-coding sequence, and 434 bp of the 3-UTR, which is followed by the human growth hormone polyadenylation signal.", "genotyping": "", "es_cell_line": "", "phenotype": "Overexpression of phospholamban was expressed in mouse slow-twitch skeletal muscle which resulted in muscle atrophy and slow rates of contraction and relaxation of the soleus muscle.", "founder_background": "FVB/N", "current_background": "FVB/N", "strain_development": "", "research_applications": "
  • Skeletal muscle contraction\n
    • \n
  • \n Calcium cycling
    • ", "donor_institution": "", "reference": "

    Song Q, Young KB, Chu G, Gulick J, Gerst M, Grupp IL, Robbins J, Kranias EG.Overexpression of phospholamban in slow-twitch skeletal muscle is associated withdepressed contractile function and muscle remodeling. FASEB J. 2004Jun;18(9):974-6. Epub 2004 Apr 1. (Medline PMID: 15059971)

    ", "addl_reference": "", "coat_color": "White", "eye_color": "Red", "other_appearance": "", "breeding_system": "Sib-mating", "breeding_schemes": "", "generation": "Unknown", "breeding_performance": "Good", "reproductive_statistics": "\n Homozygotes are viable:\n Unknown \n Unknown \n \n \n Homozygotes are fertile:\n Unknown \n Unknown \n \n \n Heterozygotes (Tg) are fertile:\n Yes \n Yes \n \n \n  \n \n \n Age Reproductive Decline:\n Unknown\n Unknown\n ", "ave_pups_born": "8", "homozgosity_yn": "Yes", "average_pups_weaned": "8", "wean_age": "3 weeks", "special_husbandry": "", "available_control": "", "parent_cell_line": "", "background_strain": "", "culture_protocol": "", "health_status_cryo": "", "availability_level": "", "hide_colony_husbandry_info": "No", "phenotype_list": "

    Mammalian Phenotype Terms:(provided by MGI)      \\u0022\\u0022Extend all MPTs
          assigned by genotype
    Tg(Myh6-Pln)2Egk/?
            FVB/N-Tg(Myh6-Pln)2Egk
    ", "f_hom_viable": "Yes", "m_hom_viable": "Yes", "f_hom_fertile": "Yes", "m_hom_fertile": "Yes", "f_het_hemi_fertile": "Yes", "m_het_hemi_fertile": "Yes", "f_age_repdec": "", "m_age_repdec": "", "update_dt": "2020-04-27 13:35:46", "user_id": "6852", "is_transgenic": "Yes", "is_sexlinked": "No", "public_display": "Yes", "public_display_dt": "2019-06-16", "show_repro_stat": "Yes", "breeding_comment": "", "sds_status": "Complete", "hom_viable_comment": "", "hom_fertile_comment": "", "het_hemi_fertile_comment": "", "protocol_status": "Needs Protocol", "donor_notes": "", "edit_notes": "", "mammalian_phenotype_id": "", "sbm_gene_symbol": "", "sbm_allele_pairs": "", "sbm_additional_allele_info": "", "esc_please_see_also": "", "esc_cell_development": "", "created_dt": "", "muga_data": "", "microbiome": "", "citation_id": "", "show_sqc_table": "", "rrid": "RRID:MMRRC_000067-MU", "alterations": [ { "locus_id": "97", "genetic_alteration_id": "61", "alteration_id": "6", "alteration_desc": "Transgene.", "mmrrc_id": "67", "cell_line_id": "902", "additional_notes": "", "presentation_order": "0", "alteration": "Transgenic", "is_standard": "1", "subtype_of": "", "imsr_alteration_cd": "TG", "note": "", "locus_role": "Transgene", "mgi_acc_id": "MGI:97622", "symbol": "Pln", "markup_symbol": "Pln", "name": "phospholamban", "chromosome": "10", "species": "Mouse", "synonyms": "PLB", "segment_type": "Gene", "component_of": "", "locus_notes": "", "external_acc_id": "MGI:97622", "resource_id": "MGI", "last_modified_dt": "2025-12-22 09:21:59", "created_dt": "", "doid_ids": "dilated cardiomyopathy 1P [DOID:0110439]|hypertrophic cardiomyopathy 18 [DOID:0110324]|centronuclear myopathy [DOID:14717]" }, { "locus_id": "3946", "genetic_alteration_id": "61", "alteration_id": "6", "alteration_desc": "Transgene.", "mmrrc_id": "67", "cell_line_id": "902", "additional_notes": "", "presentation_order": "0", "alteration": "Transgenic", "is_standard": "1", "subtype_of": "", "imsr_alteration_cd": "TG", "note": "", "locus_role": "Promoter", "mgi_acc_id": "MGI:2155600", "symbol": "Myh7", "markup_symbol": "Myh7", "name": "myosin, heavy polypeptide 7, cardiac muscle, beta", "chromosome": "14", "species": "Mouse", "synonyms": "Myhc-b|Myhcb|beta-MHC|B-MHC|MYH-beta/slow|MyHC-I|betaMHC", "segment_type": "Gene", "component_of": "", "locus_notes": "", "external_acc_id": "MGI:2155600", "resource_id": "MGI", "last_modified_dt": "2025-12-22 09:21:59", "created_dt": "", "doid_ids": "autosomal dominant hyaline body myopathy [DOID:0111269]|autosomal recessive hyaline body myopathy [DOID:0111268]|cardiomyopathy [DOID:0050700]|congenital heart disease [DOID:1682]|dilated cardiomyopathy [DOID:12930]|dilated cardiomyopathy 1S [DOID:0110454]|distal myopathy 1 [DOID:0070197]|hypertrophic cardiomyopathy [DOID:11984]|hypertrophic cardiomyopathy 1 [DOID:0110307]|hypoplastic left heart syndrome [DOID:9955]|left ventricular noncompaction [DOID:0060480]" }, { "locus_id": "35851", "genetic_alteration_id": "61", "alteration_id": "6", "alteration_desc": "Transgene.", "mmrrc_id": "67", "cell_line_id": "902", "additional_notes": "", "presentation_order": "0", "alteration": "Transgenic", "is_standard": "1", "subtype_of": "", "imsr_alteration_cd": "TG", "note": "", "locus_role": "Transgene", "mgi_acc_id": "MGI:3834218", "symbol": "Tg(Myh7-Pln)2Egk", "markup_symbol": "Tg(Myh7-Pln)2Egk", "name": "transgene insertion 2, Evangelia G Kranias", "chromosome": "unknown", "species": "Mus musculus (mouse)", "synonyms": "PlnOE|Tg(Pln)2Egk|Tg(Myh6-Pln)2Egk", "segment_type": "Transgene", "component_of": "35853", "locus_notes": "", "external_acc_id": "MGI:3834218", "resource_id": "MGI", "last_modified_dt": "2026-02-08 04:37:43", "created_dt": "", "doid_ids": "" } ] }